Qualitative Tests of Carbohydrates Essay - 1238 Words

QUALITATIVE TESTS OF CARBOHYDRATES Final Lab Report Submitted by Brittany Fitzgerald 545301 Prepared for Nancy Cook Chemistry 3501 Monday November 26, 2012 RESULTS PART A: As a result of the Benedict’s test on various sugar solutions, it was found that galactose, mannose, arabinose, ribose, lactose, fructose, maltose, and cellobiose tested positively and therefore are considered reducing sugars. Glucose, starch, sucrose, and methyl-D-glucopyranoside on the other hand tested negatively and therefore are considered non-reducing sugars. Table 1. Results of Benedict’s test on various sugar solutions Sugar Solution | Color | Clarity | Glucose | Bright blue with orange tint on top | Clear | Galactose | Brown†¦show more content†¦These sugar solutions have the ability to reduce the copper (II) ions found in the Benedict’s reagent to produce copper (I) oxide, resulting in an alteration in color. Glucose, starch, sucrose, and methyl-D-glucopyranoside on the other hand tested negatively and therefore are considered non-reducing sugars. PART B: Seliwanoff’s test uses 6M hydrochloric acid as the dehydrating acid that differentiates a ketohexose from an aldohexose. A ketohexose (a 6-carbon sugar containing a ketone), such as fructofuranose dehydrates rapidly to yield 5-hydroxymethylfurfural, whereas an aldohexose (a 6-carbon sugar containing an aldehyde) on the other hand reacts more slowly but ultimately results in the same overall product (Mount Saint Vincent University, 2012). Once the 5-hydroxymethylfurfural is produced it reacts with the resorcinol (a condensation reagent) of Seliwanoff’s reagent to form a deep rep condensation product. The colors and the rates in which they form are used to differentiate carbohydrates between ketoses and aldoses. As a result of the Seliwanoff’s test on various carbohydrates, it was found that only fructose and sucrose created a change in color. Sucrose gives a positive test as it is a disaccharide that consists of both fructose and glucose, therefore the fructoseShow MoreRelatedDetermination Of Blood Glucose Levels And Qualitative Carbohydrate Tests1507 Words   |  7 PagesPractical Report (Determination of blood glucose levels and qualitative carbohydrate tests) Introduction: The term ‘’Carbohydrates’’ is defined as the carbon compounds which contains hydroxyl groups in large quantities (King, 2013). Carbohydrates are those organic compounds which have the empirical formula CnH2nOn, or (CH2O)n (Fromm 1997). Carbohydrates are one of the four types of organic compounds in living cells that are produces during photosynthesis and are the important source of energy inRead MoreLab Report On Carbohydrates1285 Words   |  6 PagesCarbohydrates are compounds consisting of carbons, hydrogens, and oxygens. 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